While duck cells still produced elongated viruses irrespective of PI3K/Akt inhibitor or mock treatment. Strikingly steroids, a complete loss of cytoskeletal actin architecture was observed in virus infected duck cells irrespective of inhibitor or mock treatment.Analysis of chicken and duck PI3K/Akt p85 sequence revealed amino acid changes that may interfere AIV NS1 binding and activation of PI3K/Akt. In particular, a highly acidic glutamic acid (E) amino acid residue at 475 position was observed across all major mammalian and avian hosts studied except ducks in which E475 residue was substituted with a non polar valine (V) residue (E475V).
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